Participation of the TRPA1 Receptor in the Antinociceptive and Anti-Inflammatory Effect of the α, β-Amyrin Mixture in Adult Zebrafish (Danio rerio)

Marnielle Rodrigues Coutinho; Antonio Wlisses da Silva; Maria Kueirislene Amâncio Ferreira; Emanuela de Lima Rebouças; Jesyka Macedo Guedes; Amanda Maria Barros Alves; Marcia Machado Marinho; Emmanuel Silva Marinho; Jane Eire Silva Alencar de Menezes; Maria Izabel Florindo Guedes; Helcio Silva dos Santosa

doi:10.29169/1927-5951.2025.15.06

Participation of the TRPA1 Receptor in the Antinociceptive and Anti-Inflammatory Effect of the α, β-Amyrin Mixture in Adult Zebrafish (Danio rerio)

Authors

Marnielle Rodrigues Coutinho State University of Ceara, Postgraduate Program in Biotechnology, Fortaleza, CE, Brazil
Antonio Wlisses da Silva State University of Ceara, Graduate Program in Natural Sciences, Fortaleza, CE, Brazil
Maria Kueirislene Amâncio Ferreira State University of Ceara, Graduate Program in Natural Sciences, Fortaleza, CE, Brazil
Emanuela de Lima Rebouças State University of Ceara, Postgraduate Program in Biotechnology, Fortaleza, CE, Brazil
Jesyka Macedo Guedes State University of Ceara, Graduate Program in Natural Sciences, Fortaleza, CE, Brazil
Amanda Maria Barros Alves State University of Ceara, Graduate Program in Natural Sciences, Fortaleza, CE, Brazil
Marcia Machado Marinho State University of Vale do Acaraú, Chemistry Course, Sobral, CE, Brazil
Emmanuel Silva Marinho State University of Ceara, Graduate Program in Natural Sciences, Fortaleza, CE, Brazil
Jane Eire Silva Alencar de Menezes State University of Ceara, Graduate Program in Natural Sciences, Fortaleza, CE, Brazil
Maria Izabel Florindo Guedes State University of Ceara, Postgraduate Program in Biotechnology, Fortaleza, CE, Brazil
Helcio Silva dos Santosa State University of Ceara, Postgraduate Program in Biotechnology, Fortaleza, CE, Brazil; State University of Ceara, Graduate Program in Natural Sciences, Fortaleza, CE, Brazil and State University of Vale do Acaraú, Chemistry Course, Sobral, CE, Brazil

DOI:

https://doi.org/10.29169/1927-5951.2025.15.06

Keywords:

Amyrin, Inflammatory, Antinociceptive, Zebrafish, TRPA1, Camphor

Abstract

Inflammatory pain is a symptom associated with different human diseases, and they are treated with drugs that have several serious side effects. The triterpene mixture of α, β-amyrin (ABAM) has already been reported with antinociceptive and anti-inflammatory effects in mice. The antinociceptive potential of ABAM possibly involves the mechanisms of opioid and vanilloid receptors. This study aimed to determine the antinociceptive and anti-inflammatory potential of ABAM in adult zebrafish by modulating TRPA1. Thus, ABAM had its antinociceptive effect investigated by the nociception model in adult zebrafish induced by formalin. Furthermore, the possible neuromodulation by the TRPA1 channel was evaluated by pre-treatment with camphor. ABAM exhibited significant antinociceptive and anti-inflammatory effects in adult zebrafish at all tested doses. These effects, potentially mediated through TRPA1 modulation, were observed without signs of toxicity over a 96-hour period.

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