Effects of Controlled Culture Conditions on Chemical Composition and Antimicrobial Activities of Mentha rotundifolia Essential Oils
DOI:
https://doi.org/10.29169/1927-5951.2022.12.14Keywords:
Acclimatization conditions, in vitro plant culture, essential oils, GC/MS, Mentha rotundifoliaAbstract
The study objectives were the determination of Mentha rotundifolia essential oils composition using GC/MS analysis and the evaluation of their antimicrobial activities. In addition, the determination of the relationships between plants acclimatization conditions, essential oils composition and antimicrobial activities.
Essential oils extracted via hydrodistillation method from wild plants and acclimatized plants in different culture conditions of Mentha rotundifolia. Five selected pathogenic microbial strains were used to evaluate EOs in vitro antimicrobial activities.
Essential oils GC/MS analysis revealed the dominance of the oxygenated monoterpene (piperitenone oxide). A significant effect of plant culture conditions in acclimatization room on essential oils composition compared to the control was observed. We also noted that antimicrobial activities of extracted essential oils from acclimatized plants were higher than those from field-grown M. rotundifolia plants.
Content of Piperitenone Oxide in acclimatized plantlets is 93.07% for plantlets cultured at 16°C and a photoperiod of 16 hours light / 8 hours dark, statistically higher than wild plants where it is around 78%. We also noted the presence of Limonene (5.7%) in plantlets grown at 35°C which is significantly different than that of the wild plants (1.55 %). Essential oils antimicrobial activities showed that the plantlets grown at 16 °C or in total darkness were more active towards the tested strains. While, the EO of plantlets cultured at 35 °C were the least active.
References
Benayad N. Essential oils extracted from Moroccan medicinal plants: effective means of pest control of stored foodstuffs. Morocco, Study report: Faculty of Sciences of Rabat 2008.
Dhifi W, Bellili S, Jazi S, Bahloul N, Mnif W. Essential oils’ chemical characterization and investigation of some biological activities: A critical review. Medicines 2016; 3(4): 25. https://doi.org/10.3390/medicines3040025
Bousbia N. Extraction and identification of some essential oils (Nigella, Coriander, Oregano, Thyme, Rosemary). Study of their antimicrobial activities. Algiers: INA 2004.
Riahi L, Elferchichi M, Ghazghazi H, Jebalid J, Ziadia S, Aouadhie C, Chogranif H, Zaoualif Y, Zoghlamia N, Mliki A. Phytochemistry, antioxidant and antimicrobial activities of the essential oils of Mentha rotundifolia L. in Tunisia. Ind Crops Prod 2013; 49: 883-889. https://doi.org/10.1016/j.indcrop.2013.06.032
Marta EG, Rosa C. Plant factories as biopharmac’s production. Curr Trends Biomedical Eng Biosci 2017; 6: 555-682. https://doi.org/10.19080/CTBEB.2017.06.555682
Harfi B, Benahmed A, Karkour L. Characterization of Origanum glandulosum Desf. essential oils collected from different culture conditions towards standardized ex situ production. J Essent Oil-Bear Plants 2019; 22(3): 838-850. https://doi.org/10.1080/0972060X.2019.1646163
Bernard T, Perinau F, Brav O, Delmas M, Gaset A. Extraction of essential oils: Chemistry and technology. Paris: Chemistry information 1988.
Benahmed A, Harfi B, Benbelkacem I, Daas A, Laouer H, Belkhiri A. In vitro propagated Mentha rotundifolia (L.) Huds and antioxidant activity of its essential oil. Curr Issues Pharm Med Sci 2018; 31(4): 204-208. https://doi.org/10.1515/cipms-2018-0038
Benahmed A, Harfi B, Belkhiri A. Biological activity of essential oils of Mentha pulegium from field-grown and acclimated in vitro plants. Curr Sci 2019; 116(11): 1897-1904. https://doi.org/10.18520/cs/v116/i11/1897-1904
Adams RP. Identification of essential oil components by gas chromatography/mass spectrometry. 4th ed. Allured Publishing Corporation, Carol Stream IL 2007.
Gochev V, Dobreva A, Girova T, Stoyanova A. Antimicrobial activity of essential oil from Rosa Alba. Biotech & Biotechnological Equip 2010; 24(sup1): 512-515. https://doi.org/10.1080/13102818.2010.10817892
Lorenzo D, Paz D, Dellacassa E, Davies P, Vila R, Canigueral S. Essential oils of Mentha pulegium and Mentha rotundifolia from Uruguay. Bras Arch Boil Technol 2002; 45(4): 519-524. https://doi.org/10.1590/S1516-89132002000600016
Benabdallah A, Boumendjel M, Aissi O, Rahmoune C, Boussaid M, Messaoud C. Chemical composition, antioxidant activity and acetylcholinesterase inhibitory of wild Mentha species from northeastern Algeria. S Afr J Bot 2018; 116: 131-139. https://doi.org/10.1016/j.sajb.2018.03.002
Bouhabila A, Zellagui A, Telci I, Maarfia S, Gherraf N. Changeability of essential oils in Algerian Mentha rotundifolia L. (Lamiaceae) growing in sub humid area. World J Environ Biosci 2018; 7(4): 45-47.
Kokkini S, Papageorgiou VP. Constituents of essential oils from Mentha X rotundifolia growing wild in Greece. Planta Med 1988; 38: 166-167. https://doi.org/10.1055/s-2006-962381
El Arch M, Satrani B, Farah A, Bennani L, Boriky D, Fechtal M, Blaghen M, Talbi M. Chemical composition and antimicrobial and insecticidal activities of Mentha rotundifolia essential oil of Morocco. Acta Bot Gall 2003; 150: 267-274. https://doi.org/10.1080/12538078.2003.10515996
Damien Dorman HJ, Kosar M, Kahlos K, Holm Y, Hiltunen R. Antioxidant properties and composition of aqueous extracts from Mentha species hybrids, varieties and cultivars. J Agric Food Chem 2003; 51: 4563-4569. https://doi.org/10.1021/jf034108k
Tripathi AK, Prajapati V, Ahmad A, Aggarwal KK; Khanuja SPS. Piperitenone oxide as toxic, repellent and reproduction retardant toward malarial vector Anopheles stephensi (Diptera: Anophelinae). J Med Entomol 2004; 41(4): 691-698. https://doi.org/10.1603/0022-2585-41.4.691
Ghoulami S, Il Idrissi A, Fkih Tetouani S. Synthèse de nouveaux hétérocycles à partir de lʼoxyde de piperiténone. Tetrahedron Lett 2001; 42(11): 2107-2109. https://doi.org/10.1016/S0040-4039(00)02355-8
Pino JA, Rosado A, Fuentes V. Essential oil of Mentha spicata (L.) from Cuba. J Essent Oil Res 1998; 10: 657-659. https://doi.org/10.1080/10412905.1998.9701001
Venskutonis PR. A chemotype of Mentha longifolia L. from Lithuania rich in piperitenone oxide. J Essent Oil Res 1996; 8: 91-95. https://doi.org/10.1080/10412905.1996.9700564
Nagell A, Hefendehl FW. Composition of the essential oil of Mentha rotundifolia. Planta Med 1974; 26(5): 1-8. https://doi.org/10.1055/s-0028-1097962
Kjonaas R, Croteau R. Demonstration that limonene is the first cyclic intermediate in the biosynthesis of oxygenated p-menthane monoterpenes in Mentha piperita and other Mentha species. Arch Biochem Biophys 1983; 220: 79-89. https://doi.org/10.1016/0003-9861(83)90389-2
Iscan G, Kirimer N, Kurkcuoglu M, Baser KHC, Demirci F. Antimicrobial screening of Mentha piperita essential oils. J Agric food Chem 2002; 50(14): 3943-3946. https://doi.org/10.1021/jf011476k
Carson CF, Riley TV. Antimicrobial activity of the major components of the essential oil of Melaleuca alternifolia. J Appl Bacteriol 1995; 78(3): 264-269. https://doi.org/10.1111/j.1365-2672.1995.tb05025.x
Teixeira B, Marques A, Ramos C, Batista I, Serrano C, Matos O, Neng NR, Nogueira JMF, Saraiva JA, Nunes ML. European pennyroyal (Mentha pulegium) from Portugal: chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil. Ind Crops Prod 2012; 36(1): 81-87. https://doi.org/10.1016/j.indcrop.2011.08.011
Ladjel S, Gherraf N, Hamada D. Antimicrobial effect of essential oils from the Algerian medicinal plant Mentha rotundifolia L. J Appl Sci Res 2011; 7(11): 1665-1667.
Downloads
Published
Issue
Section
License
Copyright (c) 2022 Amira Benahmed, Khaoula Cherouana, Imen Benchikhelhocine, Assia Ikhlef, Asma Meghezzi, Boualem Harfi
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Similar Articles
- Sebua Silas Semenya , Sekgothe Mokgoatšana , Alfred Maroyi, Exploration of Plant Species Used by Bapedi Ethnic Group for Ethnoveterinary Purposes: A Case Study of Ga-Mphahlele Region in the Limpopo Province, South Africa , Journal of Pharmacy and Nutrition Sciences : Vol. 9 No. 3 (2019)
- S. Shireen, A.S. Zarena, A Generalized Review on Extraction of Biomolecules , Journal of Pharmacy and Nutrition Sciences : Vol. 12 (2022)
- N. Lonigro, E. Martello, F. Perondi, A. Melocchi, A. Testa, N. Bruni, Evaluating the Protective Effects of a Pet Supplement Containing Calcium Carbonate, Magnesium Hydroxide and Natural Ingredients (Gastrik Pet®, Candioli S.r.l.) on Gastric Cells and Cytotoxicity Reduction under Hyperacidity Conditions: An In Vitro Study , Journal of Pharmacy and Nutrition Sciences : Vol. 15 (2025)
- N. Lonigro, E. Martello, F. Perondi, M. Bigliati, Z. R. Mohammed, A. Costale, E. Rosso, N. Bruni, Assessing the Efficacy of Natural Pet Products in Protecting Gastric Cells and Reducing Cytotoxicity under Hyperacidity Conditions: An In Vitro Study , Journal of Pharmacy and Nutrition Sciences : Vol. 14 (2024)
- Mohammad Rashedul Islam, Nawreen Monir Proma, Jannatul Naima, Md. Giash Uddin, Syeda Rubaiya Afrin, Mohammed Kamrul Hossain, Assessment of the Pharmacological Activities of Ardisia solanacea Roxb: An Ethnomedicinal Plant used in Bangladesh , Journal of Pharmacy and Nutrition Sciences : Vol. 10 No. 5 (2020)
- Mohammed Seid, Aman Dekebo, Neelaiah Babu, Phytochemical Investigation and Antimicrobial Evaluation of Foeniculum vulgare Leaves Extract Ingredient of Ethiopian Local Liquor , Journal of Pharmacy and Nutrition Sciences : Vol. 8 No. 1 (2018)
- Bianca I. Mergler, Ewgenia Roth, Sylvaine F.A. Bruggraber, Jonathan J. Powell, Dora I.A. Pereira, Development of the Caco-2 Model for Assessment of Iron Absorption and Utilisation at Supplemental Levels , Journal of Pharmacy and Nutrition Sciences : Vol. 2 No. 1 (2012)
- Rashidi Othman , Norazian Mohd. Hassan , Ainaa Eliah Abu Bakar , Nur Hidayah Noh , Nurrulhidayah Ahmad Fadzillah , Noraini Mahmad, Manipulation of Environmental Stress Towards Lutein Production in Chlorella fusca Cell Culture , Journal of Pharmacy and Nutrition Sciences : Vol. 9 No. 5 (2019)
- A.F. Ogundola, T.A. Yekeen, R.A. Arotayo, A.O. Akintola, A.O. Ibrahim, H.O. Adedosu, M.O. Bello, Evaluation of Nutrients in Leaves and Seeds of Calotropis Procera (linn) , Journal of Pharmacy and Nutrition Sciences : Vol. 11 (2021)
- Md. Sahab Uddin , G.M. Sala Uddin , Mst. Marium Begum , Yesmin Begum , Oscar Herrera-Calderon , Md. Manjurul Islam , Mohamed M. Abdel-Daim, Inspection of Phytochemical Content and In Vitro Antioxidant Profile of Gnaphalium luteoalbum L.: An Unexplored Phytomedicine , Journal of Pharmacy and Nutrition Sciences : Vol. 7 No. 3 (2017)
You may also start an advanced similarity search for this article.