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Effect of Temperature on the Chemical Quality of β-Carotene Extracted from Azollafiliculoides of Anzali Wetland

Authors

  • Mina Seifzadeh

    National Fish Processing Research Center, Inland Waters Aquaculture Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Anzali, Iran

  • Ali Raoufi

    Student Research Committee, Department of Medicine, Faculty of Medicine, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran

DOI:

https://doi.org/10.29169/1927-5951.2025.15.08

Keywords:

Anzali wetland, A filiculoides, Food hygiene, Natural pigment, Season

Abstract

Purpose: This study assesses the quality of β-Carotene from a natural source, Azolla, in comparison with synthetic β-Carotene, and looks at seasonal variation in composition.

Methods: Azolla collected from summer and winter was extracted using organic solvents. The synthetic β-Carotene used as a control was obtained from a pharmacy. All treatments were stored for one year at 5 oC. The samples were analyzed to measure purity and concentration, and then used for colorimetric and vitamin A analysis.

Main findings: Our results showed significant differences (p< 0.05) between experimental and control treatments. The winter Azolla samples contained larger amounts of the β-Carotene (p <0.05) than the β-Carotene samples from summer Azolla which contain β-Carotene but in lesser amounts than winter samples. Tetrahydrofuran provided the best β-Carotene solubility, and methanol and acetonitrile the lowest. Cyclohexanone provided the most degradation. After one year of storage, the experimental treatments did, however, retain a reasonable acceptable chemical quality.

Conclusion: Considering the health benefits of natural β-Carotene over synthetic β-Carotene, this study indicated β-Carotene extracted from Azolla could be a viable alternative to synthetic β-Carotene in the food industry. 

References

[1] MacDougall DB. Color in Food: Improving Quality. England: Woodhead Publishing; 2002: 392.

[2] Stahl U, Donalies UEB, Nevoigt UEBU. Food Biotechnology. Germany: Springer; 2008: 269.

[3] Branen AL, Davidson M, Salminen SP, Thorngate J. Food Additives. Virginia: Taylor and Francis; 2001: 952-61.

[4] Hopkins WG. Plant Biotechnology. United States: Infobase Publishing; 2006: 365.

[5] Rymbai H, Sharma RR, Srivastav M. Biocolorants and its implications in health and food industry: A review. Int J Pharm Technol Res 2001; 3: 2228-44.

[6] Caivano JL, Buera MDP. Color in Food: Technological and Psychophysical Aspects. Florida: CRC Press; 2012: 132.

[7] Cosma P, Fini P, Rochira S, Catucci L, Castagnolo M, Agostiano A. Phototoxicity and cytotoxicity of chlorophyll a/cyclodextrins complexes on Jurkat cells. Bioelectrochem 2008; 74: 58-64.

[8] Schieber A, Carle R. Occurrence of carotenoid cis-isomers in food: Technological, analytical, and nutritional implications. Trends Food Sci Technol 2005; 16: 416-22.

[9] Hutchings JB. Food Color and Appearance. Gaithersburg, Md: Aspen Publishers; 2003. p. 273.

[10] Agostiano A, Catucci L, Cosma P, Fini P. Aggregation processes and photophysical properties of chlorophyll a in aqueous solutions modulated by cyclodextrins. J Phys Chem 2003; 5: 2122-813.

[11] Hosotani K, Kitagawa M, Granado F, Olmedilla B, Gilmantines E, Blando I. A fast reliable and low cost saponification protocol for analysis of carotenoids in vegetables. J Food Compos Anal 2001; 14: 479-85.

[12] Achikanu CE, Eze-Steven PE, Ude CM, Ugwuokolie OC. Determination of the vitamin and mineral composition of common leafy vegetables in southeastern Nigeria. Int J CurrMicrobiolAppl Sci 2013; 2: 347-53.

[13] Blake JA, Moran JJ. An improved colorimetric procedure for the analysis of vitamin A. Can J Chem. 1976; 54: 1757-64.

[14] Budowski P, Bondi A. Determination of vitamin A by conversion to anhydrovitamin A. Analyst. 1957; 82: 751-9.

[15] Muller H. Determination of the carotenoid content in selected vegetables and fruit by HPLC and photodiode array detection. Z LebensmUntersForsch. 1997; 204: 88-94.

[16] Schierle J, Pietsch B, Ceresa A, Fizet C. Method for the determination of carotene in supplements and raw materials by reversed-phase liquid chromatography: Single laboratory validation. J AOAC Int 2004; 87: 1070-82.

[17] Khalil IA, Varananis FR. Carotenoid extraction and analysis by reversed phase HPLC system. Sarhad J Agric. 1996; 105: 15-21.

[18] Craft NE. Relative solubility, stability, and absorptivity of lutein and β-carotene in organic solvents. J Agric Food Chem. 1992; 40: 431-4.

[19] Vieira DAP, Caliari M, Souza ERB, Junior MSS. Methods for pigment extraction and determination of color in tomato processing cultivars. Food Sci Technol 2018; 3: 1-7.

[20] Davies BH. Carotenoids. In: Goodwin TW, editor. Chemistry and Biochemistry of Plant Pigments. England: Academic Press; 1976. p. 155.

[21] International Commission on Illumination (CIE). The Effect of Spectral Power Distribution on Lighting for Urban and Pedestrian Areas. Austria: CIE; 2014. p. 78.

[22] AOAC. Official Methods of Analysis Manual. 18th ed. United States: AOAC; 2005. p. 123.

[23] Amaya R. The effect of processing and storage on carotenoids content of vegetables. J Food Sci 2001; 7: 20055802.

[24] Anjum F, Barkat AK, Noreen N, Tariq M, Faisal S. Effect of boiling and storage on beta-carotene content of different vegetables. Pak J Life Soc Sci 2008; 6: 63-7.

[25] Çinar I. Carotenoid pigment losses of freeze-dried plant samples under different storage conditions. Food Sci Technol 2004; 37: 363-7.

[26] Araus KA, Canales RI, Valle JM, Fuente JC. Solubility of β-carotene in ethanol- and triolein-modified CO₂. J ChemThermodyn 2011; 43: 1991-2001.

[27] Venugopal V, Prasanna P, Sood A, Jaiswal P, Kaushik BD. Stimulation of pigment accumulation in Anabaena-Azolla strains: effect of light intensity and sugars. Folia Microbiol 2006; 51: 50-6.

[28] Qiu D, Chen ZR, Li HR. Qualitative analysis of β-carotene isomers. Food Sci 2008; 29: 50-3.

[29] Hackett M, Lee J, Schwartz S. Thermal stability and isomerization of lycopene in tomato oleoresins from different varieties. J Food Sci 2002; 69: 536-41.

[30] Khanipour E, Keramat J, HosseiniParvar SH, Motamedzadegan A, Ghorbani A, Shahidi SA. Application of extracted tomato carotenoids in heated and unheated foods: Study of color stability during storage. Iran J Food Sci Technol 2006; 2: 13-22.

[31] Prasanna R, Pabby A, Singh PK. Effect of glucose and light/dark environment on pigmentation profiles in Calothrixelenkeni. Folia Microbiol 2004; 49: 26-30.

[32] Bendich A, Higdon GS. Biological actions of beta-carotene. Am J Nutr 2004; 32: 225-30.

[33] Alcaíno A, Barahona S, Carmona M, Lozano C, Marcoleta A, Iklitschek M, et al. Cloning of the cytochrome P450 reductase (crtR) gene and its involvement in the astaxanthin biosynthesis of Xanthophyllomycesdendrorhous. BMC Microbiol 2008; 8: 1-13.

[34] Dentuto PL, Catucci L, Cosma P, Fini P, Agostiano A, Hackbarth S. Cyclodextrin/chlorophyll a complexes as supramolecular photosensitizers. Bioelectrochem 2007; 70: 39-43.

[35] Mercandate A, Amaya R. Carotenoid composition and vitamin A value of some native Brazilian green leafy vegetables. J Food Sci. 1990; 25: 213-9.

[36] Agte VV, Tarwadi KV, Mengale S, Chiplonkar SA. Potential of traditionally green leafy vegetables as natural sources for supplementation of eight micronutrients in vegetarian diets. J Chromatogr 2000; 13: 885-91.

[37] Chromatogr BJ. Improved simultaneous determination method of beta-carotene and retinol with saponification in human serum and rat liver. Anal Technol Biomed Life Sci 2003; 1: 305-13.

[38] Negi P, Roy S. Effect of drying condition on quality of green leaves during long-term storage. Food Res Int 2000; 34: 283-7.

[39] Yamini C, Ranjana N, Chaturvedi Y, Nagar R. Levels of beta-carotene and effects of processing on selected fruits and vegetables of the arid zone of India. J Food Sci 2001; 56: 7-32.

[40] Zareh D, Kiani Rad M. Comparison of beta-carotene production by Blakesleatrispora fungi in fifteen liter fermenter and seventy-five-liter airlift. Res Const 2004; 64: 2-7.

[41] Razavi H, Rezaei K, Mark I. Comparison of pigment extraction (carotenoids) methods from Sporobolomycesruberrimus H110 yeast. Res Food SciTechnol Iran 2005; 1: 33-42.

[42] Baigan E, Rasekh F, Rasekhi N. Production and extraction of carotenoids from living organisms. In: National Conference on New Findings of Chemistry in Industry and Medicine; Iran: Islamic Azad Univ. Ray Branch; 2009. p. 156.

[43] Moghadassi Z, Emtiazjoo M, Rabanie M, Emtiazjoo M, Azargashb E, Mosaff N. Potential β-carotene production from Dunaliellasalina of Shahe Lake under salinity stress. J Fish Islam Azad UnivAzadshahr Br 2011; 5: 90-101.

[44] Moghadassi Z, Emtyazjoo M, Rabanie M, Emtyazjoo M, Labibie F, Azarghashb E, et al. Study of anti-cancer ethanol extract of Dunaliellasalina isolated from Hoz-Soltan against squamous cell carcinoma in vitro. Iran J Med Aromat Plants 2011; 27: 306-15.

[45] Lejeune A, Peng J, Boulenge EL, Larondelle Y, Hove CV. Carotene content of Azolla and its variations during drying and storage treatments. Anim Feed Sci Technol 2000; 84: 293-301.

[46] Mustafa EM, Ibrahim MM. HPLC analysis of non-enzymatic antioxidants in Azollacaroliniana (Pteridopsida) subjected to UV-B. Biol Sci 2012; 3: 19-30.

[47] Anguelova T, Warthesen Y. Degradation of lycopene, α-carotene and β-carotene during lipid peroxidation. J Food Sci 2000; 65: 71-5.

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Published

2025-07-18

Issue

Vol. 15 (2025)

Section

Articles

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How to Cite

Effect of Temperature on the Chemical Quality of β-Carotene Extracted from Azollafiliculoides of Anzali Wetland. (2025). Journal of Pharmacy and Nutrition Sciences , 15, 76-84. https://doi.org/10.29169/1927-5951.2025.15.08

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