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Effect of Temperature on the Chemical Quality of β-Carotene Extracted from Azollafiliculoides of Anzali Wetland

Authors

  • Mina Seifzadeh

    National Fish Processing Research Center, Inland Waters Aquaculture Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Anzali, Iran

  • Ali Raoufi

    Student Research Committee, Department of Medicine, Faculty of Medicine, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran

DOI:

https://doi.org/10.29169/1927-5951.2025.15.08

Keywords:

Anzali wetland, A filiculoides, Food hygiene, Natural pigment, Season

Abstract

Purpose: This study assesses the quality of β-Carotene from a natural source, Azolla, in comparison with synthetic β-Carotene, and looks at seasonal variation in composition.

Methods: Azolla collected from summer and winter was extracted using organic solvents. The synthetic β-Carotene used as a control was obtained from a pharmacy. All treatments were stored for one year at 5 oC. The samples were analyzed to measure purity and concentration, and then used for colorimetric and vitamin A analysis.

Main findings: Our results showed significant differences (p< 0.05) between experimental and control treatments. The winter Azolla samples contained larger amounts of the β-Carotene (p <0.05) than the β-Carotene samples from summer Azolla which contain β-Carotene but in lesser amounts than winter samples. Tetrahydrofuran provided the best β-Carotene solubility, and methanol and acetonitrile the lowest. Cyclohexanone provided the most degradation. After one year of storage, the experimental treatments did, however, retain a reasonable acceptable chemical quality.

Conclusion: Considering the health benefits of natural β-Carotene over synthetic β-Carotene, this study indicated β-Carotene extracted from Azolla could be a viable alternative to synthetic β-Carotene in the food industry. 

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Published

2025-07-18

Issue

Vol. 15 (2025)

Section

Articles

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How to Cite

Effect of Temperature on the Chemical Quality of β-Carotene Extracted from Azollafiliculoides of Anzali Wetland. (2025). Journal of Pharmacy and Nutrition Sciences , 15, 76-84. https://doi.org/10.29169/1927-5951.2025.15.08

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