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Phytochemical Screening and Antimicrobial Activity of Entandrophragma angolense

Authors

  • Anthonia Ogheneruno Ugboduma

    Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

  • Francis Adu

    Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

  • Christian Agyare

    Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

  • Kofi Annan

    Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

  • Samuel Osei-Asante

    Kumasi Polytechnic, Kumasi, Ghana

DOI:

https://doi.org/10.6000/1927-5951.2013.03.04.5

Keywords:

 Phytochemical screening, antibacterial, antifungal, time kill kinetics.

Abstract

Entandrophragma angolense (Welw.) C.DC. (Meliaceae) is a deciduous plant commonly found on in west Africa and it is used for treatment microbial infections, wounds, rheumatic and arthritic pains. The methanol stem bark extract was investigated for its antimicrobial activity using the agar well diffusion method and macro-dilution technique and time kill kinetics. The antimicrobial activity of the methanol extract and its fractions were evaluated against five bacteria species and a fungus including Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Bacillus subtilis NCTC 10073, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 4853 and clinical strains of Candida albicans and some of the test bacteria. Phytochemical screening of the crude extract revealed the presence of tannins, flavonoids, alkaloids, terpenoids, saponins and cardiac glycosides. The MICs of the methanol extract against the test organisms were: S. aureus (11.0 mg/mL), E. faecalis (9.0 mg/mL), B. subtilis (13.0 mg/mL) E. coli (17.0 mg/mL), P. aeruginosa (19 mg/mL) and C. albicans (15.0 mg/mL). The fractions of the methanol extract (pet ether, ethyl acetate and aqueous fractions) exhibited varying antimicrobial activities, with the highest activity exhibited by the aqueous fraction. The extract exhibited bacteriostatic and fungistatic activity against all test organisms. Time kill studies showed that the extract exhibited an inhibition of bacterial and fungal growth. The results indicate that the methanol stem bark of E. angolense has antibacterial and antifungal activities and may justify the medicinal uses of the plant in the management of bacterial and fungal infections.

References

Robbers J, Speedie M, Tyler V. Pharmacognosy and pharmaco biotechnology. Williams and Wilkins, Baltimore 1996; pp. 1-14.

Oluwatuyi M, Kaatz GW, Gibbons S. Antibacterial and resistance modifying activity of Rosmarinus offinalis. Phytochemistry 2004; 65(24): 3249-54. http://dx.doi.org/10.1016/j.phytochem.2004.10.009

Borris RP. Natural products research: perspectives from a major pharmaceutical company. J Ethnopharmacol 1996; 51: 29-38. http://dx.doi.org/10.1016/0378-8741(95)01347-4

Akinyemi KO, Oladapo O, Okwara CE, Ibe CC, Fasure AK. Screening of crude extracts of some medicinal plants used in south-west Nigerian unorthodox medicine for anti-methicilin resistant Staphylococcus aureus. BMC Compl Altern Med 2005; 11: 5-6.

Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol 2005; 4: 685-88.

Irvine FR. Woody plants of Ghana. Oxford University Press, London 1961; pp. 48-50.

Obih PO, Makinde JM, Laoje J. Investigation of various extracts of Morinda lucida for antimalarial actions on Plasmodium berghei in mice. Afr J Med Sci 1986; 14: 45-49.

Bray DH, Warhurst DC, Connolly JD, O’Neill MJ, Phillipson JD. Plants as sources of antimalarial drugs. Part 7. Activity of some species of Meliaceae and their constituent limonoids. Phyto Res 1990; 4: 29-35. http://dx.doi.org/10.1002/ptr.2650040108

Njar VCO, Adesanwo JK, Raji Y. Methyl angolensate: The antiulcer agent of the stem bark of Entandrophragma angolense. Planta Med 1994; 61(1): 91-92. http://dx.doi.org/10.1055/s-2006-958015

Orisadipe A, Amos S, Adesomoju A, Binda L, et al. Spasmolytic activity of methyl angolensate: a triterpenoid isolated from Entandrophragma angolense. Biol Pharm Bull 2001; 24(4): 364-67. http://dx.doi.org/10.1248/bpb.24.364

Bickii J, Tchouya GRF, Tsamo E. The antiplasmodial agents of the stem bark of Entandrophragma angolense (Meliaceae). Afr J Tradit Complement Altern Med 2007; 4(2): 135-39.

Tchouya GRF, Bickii J, Barhé TA, Boyom FF, Djakou BL, Tchouankeu JC. Antiplasmodial activities of limonoids from Entandrophragma angolense (Meliaceae), and their semi-synthetic derivatives: Study of the structure-activity relationship. Spatula DD 2013; 3(2): 45-50. http://dx.doi.org/10.5455/spatula.20130516064018

Sofowara A. Screening plants for bioactive agents in medicinal plants and traditional medicine in Africa. 2nd ed. Spectrum Books Ltd., Sunshine House, Ibadan 1993; pp. 134-156.

Trease GE, Evans WC. Pharmacognosy. 15th ed., Saunders Publishers, London 2002; pp. 214-393.

Harborne JB. Phytochemical methods: A guide to modern techniques of plant analysis. Chapman and Hall, London 1998; pp. 182-190.

Rauha JP, Remes S, Heinonen M, Hopia A, Kähkönen M, Kujala T, et al. Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. Int J Food Microbiol 2000; 56(1): 3-12. http://dx.doi.org/10.1016/S0168-1605(00)00218-X

Akinpelu DA, Kolawale DO. Phytochemistry and antimicrobial activity of leaf extract of Piliostigma thonningii (Schum). Sci Foc 2004; 7: 64-70.

Aibinu I, Adenipekun T, Adelowotan T, Ogunsanya T, Odugbemi T. Evaluation of the Antimicrobial Property of different parts of Citrus aurantifoloa (Lime fruit) as used locally. Afr J Tradit Complement Altern Med 2007; 4(2): 185-90.

Adeniyi BA, Fong HH, Pezzuto JM, Luyengi L, Odelola HA. Antibacterial activity of diospyrin, isodiospyrin and bisisodiospyrin from Diospyros piscatoria Gurke (Ebenaceae). Phyto Res 2000; 14: 112-17. http://dx.doi.org/10.1002/(SICI)1099-1573(200003)14:2<112::AID-PTR488>3.0.CO;2-T

Cuhnie TP, Lamb AJ Antimicrobial activity of flavonoids. Int J Antimicrob Agent 2006; 26: 342-56.

Hendrich AB. Flavonoid-membrane interactions: possible consequences for biological effects of some polyphenolic compounds. Acta Pharmacol Sin 2006; 27(1): 27-40. http://dx.doi.org/10.1111/j.1745-7254.2006.00238.x

Trombetta D, Astelli F, Grazia-Sarpietro M, Venuti V, Cristani M, Daniele C, et al. Mechanisms of antibacterial action of three monoterpenes. Antimicrob Agents Chemother 2005; 49: 2474-78. http://dx.doi.org/10.1128/AAC.49.6.2474-2478.2005

Ahmadu AA, Akputu IN, Hassan HS, Sule MI, Pateh UU. Preliminary phytochemical and antimicrobial screening of the leaves of Byrsocarpas coccineus Schun and Thonn. (Connaraceae). J Pharm Biores 2006; 3(2): 107-10.

Fabry W, Okemo PO, Ansorg R. Antibacterial activity of east African medicinal plants. J Ethnopharmacol 1998; 60: 79-84. http://dx.doi.org/10.1016/S0378-8741(97)00128-1

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Published

2013-08-05

Issue

Vol. 3 No. 4 (2013)

Section

Articles

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Copyright (c) 2013 Anthonia Ogheneruno Ugboduma, Francis Adu, Christian Agyare, Kofi Annan , Samuel Osei-Asante

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

Phytochemical Screening and Antimicrobial Activity of Entandrophragma angolense. (2013). Journal of Pharmacy and Nutrition Sciences , 3(4), 241-249. https://doi.org/10.6000/1927-5951.2013.03.04.5

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