Effect of Fermentation on Nutrient and Antinutrient Contents of Cocoyam Corm


  • Adegbehingbe Kehinde Tope Adekunle Ajasin University, Akungba-Akoko, Nigeria
  • Fakoya Soji Ondo State University of Science and Technology, Okitipupa, Nigeria




 Cocoyam, fermentation, nutrient, antinutrient, phytochemicals.


Objective: Cocoyam corms were fermented with the aim of enhancing and reducing its nutrient and antinutrient contents respectively.

Methods: Cocoyam corm was fermentated naturally by submerged fermentation method in a sterile medium (distilled water) for four days. Microbial examination of the fermenting corms was carried out at 24hours interval for four days.

Results: Twenty bacterial strains were isolated within the fermentation periods. They include the general: Micrococcus species, Lactobacillus plantarum, L. fermentum, Enterobacter, Escherichia coli and Staphylococcus aureus. The total bacteria count increased from 5.70 log cfu/ml to 8.97 log cfu/g while fungal count increased from 3.33 log cfu/g to 4.84 log cfu/g. Temperature and the total titratable acidities increased from 27oC to 35oC and 1.13% to 3.72% respectively while the pH values decreased from 5.68 to 3.75. The result of the proximate analysis showed that the fermented sample had higher protein (12.00%), ash (2.84%) and fat (4.84%) contents than the unfermented sample which contained 7.30%, 2.4% and 4.55% respectively. However, moisture, fibre and carbohydrate contents decreased from 9.70%, 3.00% and 73.04% in unfermented sample to 8.94%, 2.78% and 67.60% in fermented sample respectively. All the antinutrient contents decreased at the end of the fermentation [phytate (1.32-0.38) g/100DM, oxalate (0.72-0.21) g/100DM, tannin (0.18-0.07) g/100DM, saponin (0.45-0.22) g/100DM, hydrocyanide (22.27-10.22)g/kg of the fermented sample than the unfermented one.


Achi OK. Microorganisms associated with natural fermentation of prospopis African seeds for the production of Okpiye. Plant Food Hum Nutr 1992; 42: 295-304. http://dx.doi.org/10.1007/BF02194090

Olajide R, Akinsoyinu AO, Babayemi OJ, Omojola AB, Abu AO,Afolabi KD. Effect of processing on energy values, nutrient and anti-nutrient components of wild cocoyam [Colocasia esculenta (L.) Schott

Falayi BA, Balogun AM, Adebayo OT, Madu CT, Eyo A. Comparison of seven locally prepared starches Nigeria with sodium carboxyl methylcellulose for water stability in African catfish (Clarias gariepinus) feeds. J Sustainable Tropical Agric Res 2004; 9: 104-8.

Aderolu AZ, Lawal MO, Oladipupo MO. Processed cocoyam tuber as carbohydrate source in the diet of juvenile African catfish (Clarias Gariepinus). Eur J Sci Res 2009; 35(Pt3): 453-60.

Opera LU. Edible aroid postharvest operation. In: AGST/FAO: Danile, M. Massey University, New Zealand 2002.

Ojinnaka MC, Akobundu ENT, Iwe MO. Cocoyam starch modification effects on functional, sensory and cookies qualities. Pak J Nutr 2009; 8(Pt5): 558-567.

Onwuka NO, Eneh OC. The potentials of cocoyam (Colocasia) in stout beer brewing. J Sci Technol 1998; 4: 78-80.

Asumugba VU, Umalaka BC. Chemical and organoleptic evaluation of snacks development from cocoyam. J Nig Agric 2002; 31: 78-88.

Eleje I. Cocoyam, a major national carbohydrate staple for the future in Nigeria. In: (Eds. Arene OB, Ene LSO, Odurukwe SO, Ezeh NOA). Proc. 1st National Workshop on cocoyams. NRCRI, Umudike, Nigeria 1987; 11-16.

Onwueme IC. Strategies for increasing cocoyam (Colocasiaand Xanthosomasp.) in Nigerian food basket. In: (Eds. Arene OB, Ene LSO, Odurukwe SO, Ezeh NOA). Proc. 1st National Workshop on Cocoyam NRCRI Umudike, Nigeria 1987; 35-42.

Hahn SK. Topical root crops their improvement and utilisation, based on Paper presented at a conference organized by the Common Wealth Agric. Bureau on Advancing Agricultural production in Africa. Arusha, Tanzania international Conference (IITA) 1984; 2: 28.

Ezedinma FOC. Response of taro (Colocasia esculenta) to water management, plot preparation and population. 3rd Intl. symp. Trop. Root Crops, Ibadan, Nigeria 1987.

Fagbenro OA, Adebayo T. A review of the animals and aquafeed industries in Nigeria. In: Livestock in livestock and fishfeeds in sub-Saharan African, compiled by tom Hechi. FAO Fisheries Technical paper, FAO, Rome 2002; 21.

Agwunobi LN, Awukam PO, Cora OO, Isika, MA. Studies on the use of Colocasia esculenta (taro cocoyam) in the Diets of weaned pigs. Trop Anim Health Production 2002; 34: 241-7. http://dx.doi.org/10.1023/A:1015234709538

Abdulrashid M, Agwunobi NI. Taro cocoyam (Colocasia esculenta) meal as feed ingredient in poultry. Pak J Nutrition 2009; 8: 668-73. http://dx.doi.org/10.3923/pjn.2009.668.673

Akpan EJ, Umoh IB. Effect of heat and tetracycline treatment on the food quality and acridity factors in cocoyam (Xanthososmas agittifolium) (L)Scholt). Pak J Nut 2004; 3: 240-3. http://dx.doi.org/10.3923/pjn.2004.240.243

Olutiola PO, Famurewa O, Sontag HG. An introduction to general microbiology. A practical Approach. 2nd edition. Heideberger Verlagsanstait and DruckereiGmb. Heldelberg Publisher 2000.

Onions AHS, Allsopp D, Eggins HOW. Smith’s Introduction to industrial Mycology. 8th ed., The pitman press, bath 1995; 65-92.

AOAC. Official methods of analysis of the Association of Official Analytical Chemists.17th edition. AOAC, VA, USA 2005; 50: 18

Egwaikhide PA, Okeniyi SO, Gimba CO. Screening for anti-microbial activity and phytochemical constituents of some Nigerian medicinal plants. J Med Plants Res 2009; 3(Pt12): 1088-91.

Bradbury MG, Egen SV, Bradbury JH. Determination of all forms of cyanogens in cassava roots and cassava products using picrate paperkits. J Sci Food Agric 1999; 79: 593-601. http://dx.doi.org/10.1002/(SICI)1097-0010(19990315)79:4<593::AID-JSFA222>3.0.CO;2-2

Roy DN, Rao PS. Evidence, isolation and some properties of trypsin inhibitor in Lathyrus sativus. J Agric Food Chem 1971; 19: 257-4.

Lucas GM, Markakes P. Phytic acid and other phosphorus compounds of nevy bean Phaseolos vulgaris). J Agric Food Chem 1975; 23: 13-5. http://dx.doi.org/10.1021/jf60197a016

Day RA, Underwood AL. Quantitive analysis 5th ed. Prentice. Hall Publication 1986; 701.

Van der poel AFB. Effect of processing on ant-nutritional factor and protein nutritional value of dry beans. Ani Feed Sci Technol 1990; 2: 179-208.

Donohue DC, Salminen S. Safety of Probiotic Bacteria. Asia Pacific J Clin Nutr 1996; 5: 25-8.

Jonathan G, Ajayi, Omitade Y. Nutritional compositions, fungi and aflatoxins detectionin stored ‘gbodo’ (fermented Dioscorea rotundata) and ‘eluboogede’ (fermented Musa parasidiaca) from Southwestern Nigeria. Afr J Food Sci 2011; 5(Pt 2): 105-10.

Agarry OO, Nkama I, Akoma O. Production of Kunun-zaki (A Nigerian fermented cereal beverage) using starter culture. Int Res J Microbiol 2010; 1(Pt 2): 018-025.

Kayode APP, Hounhouigan JD, Nout MJR. Impact of brewing process operations on phytate, phenolic compunds and in vitro solubility of iron and zinc in opaque sorghum beer. LWT- Food Sci Technol 2007; 40: 834-41.

Dike KS, Sanni AI. Influence of starter culture of lactic acid bacteria on the shelf life of agidi, an indigenous fermented cereal. Afr J Technol 2010; 9(Pt46): 7922-7.

Lyumugabe F, Kamaliza G, Bajyana E, Thonart PH. Microbiological and physico-chemical characteristic of Rwandese traditional beer “Ikigage”. Afri J Biotechnol 2010; 9(Pt 27): 4241-6.

Holzapfel W. Use of starter cultures in fermentation on a household scale. Food Control 1997; 8: 241-58. http://dx.doi.org/10.1016/S0956-7135(97)00017-0

Munyaja MBK, Narvhus JA, Treimos J, Langsrud T. Isolation, characterization and identification of lactic acid bacteria from bushera: Ugandan traditional fermented beverage. Int J Food Microb 2003; 8: 201-10. http://dx.doi.org/10.1016/S0168-1605(02)00148-4

Achi OK. Quality attributes of fermented yam flour supplemented with processed soy flour. Plant Food Human Nut 1999; 54: 151-8. http://dx.doi.org/10.1023/A:1008130218830

Teniola OD, Odunfa SA. Microbial assessment and quality evaluation of ogi during spoilage. World J Micro Biotechnol 2002; 18: 731-7. http://dx.doi.org/10.1023/A:1020426304881

Wakil SM, Kazeem MO. Quality assessment of weaning food produced from fermented cereal-legume blends using starters. Inter Food Res J 2012; 19(Pt4): 1679-85.

Lyumugabe F, Kamaliza G, Bajyana E, Thonart PH. Microbiological and physico-chemical characteristic of Rwandese traditional beer “Ikigage”. Afr J Biotechnol 2010; 9(Pt 27): 4241-6.

Audu OJ. Comparative studies of wine produced from spontaneous and controlled fermentation of cashew (Anacarium occidentale) juice. Res J Biological Sci 2012; 5(Pt 7): 460-2.

Marktam RAB, Sarkar PK. Survival and growth of food borne bacterial pathogen in fermenting dough of wadi-a legume based indigenous food. J Food Sci Tech 2010; 0185-0192.

Oboh G, Elusiyan CA. Changes in the nutrient and anti-nutrient content of micro-fungi fermented cassava flour produced from low- and medium-cyanide variety of cassava tubers. Afr J Biotechnol 2007; 6(Pt 18): 2150-7.

Oboh G, Akindahunsi AA. Biochemical changes in Cassava products (flour &gari) subjected to Saccharomyces cerevisiae solid media fermentation. Food Chem 2003; 82(Pt4): 599-602. http://dx.doi.org/10.1016/S0308-8146(03)00016-5

Oyarekua MA. Biochemical and Microbiological changes during the production of fermented pigeon pea (Cajanus cajan) flour. Afr J Food Sci Tech 2011; 2(Pt10): 223-31.

Onoja US, Obizoba IC. Nutrient composition and organoleptic attributes of gruel based on fermented cereal, legume, tuber and root flour. Agro-Science Journal of Tropical Agriculture, Food, Environment and Extension. 2009; 8(Pt 3): 162 -8.

Mugendi JB, Njagi ENM, Kuria EN, Mwasaru MA, Mureithi JG, Apostolides Z. Effects of processing technique on the nutritional composition and anti-nutrient content of mucuna bean (Mucunapruriens L.). Afr J Food Sci 2010; 4(Pt4): 156-66.

Onweluzo JC, Nwabugwu CC. Fermentation of millet (Pennisetum americanum) and pigeon pea (Cajanus cajan) seeds for flour production: Effects on composition and selected functional properties. Pak J Nut 2009; 8(Pt6): 737-44.

Fardiaz D, Markakis P. Degradation of phytic acid in oncom (fermented peanut press cake). J Food Sci 1981; 46: 523-5. http://dx.doi.org/10.1111/j.1365-2621.1981.tb04901.x

Effiong OO, Umoren UE. Effects of multiprocessing techniques on the chemical composition of horse eye beans (Mucuna urens). Asian J Animal Sci 2011; 5(Pt 5): 340-8. http://dx.doi.org/10.3923/ajas.2011.340.348

Montgomery RD. Cyanogens in I. E. Liener (Eds.) Toxic constituents of plant food stuffs. Academic Press, New York 1980; 143-157.

Akande KE, Doma UD, Agu HO, Adamu HM. Major anti-nutrients found in plant protein sources: Their effect on nutrition. Pak J Nut 2010; 9: 827-32. http://dx.doi.org/10.3923/pjn.2010.827.832

D’Mello JPF. Anti-nutritional factors and mycotoxins. In: Farm animal metabolism and nutrition. CAB International Wallingford, UK 2000; 383-403. http://dx.doi.org/10.1079/9780851993782.0383

Akinrele IA, Cook AS, Holgate RA. The manufacture of gari from cassava in Nigeria. In Proceeding of 1st International Congress of Food Technology, London1962; 633-44.

Oboh G, Akindahunsi AA, Oshodi AA. Nutrient and Anti-nutrientcontent of Aspergillus niger fermented Cassava Products (flour and gari). J Food Compost Anal 2002; 15(5): 617-22.

Obizoba IC, Atii JV. Effects of soaking, sprouting, fermentation and cooking on nutrient composition and some anti-nutritional factors of sorghum (guinesia) seeds. Plant Foods Human Nut 1991; 41: 203-12. http://dx.doi.org/10.1007/BF02196388

Nnam NM, Obiakor PN. Effect of fermentation on the nutrient and antinutrient composition of Baobab (Adansonia digitata) seeds and rice (Oryza sativa) grains. Eco Food Nut 2003; 42: 265-70.

Jimoh WA, Fagbenro OA, Adeparusi EO. Effect of processing on some minerals, anti-nutrientsand nutritional composition of sesame (Sesamum indicum) seed meals. Electronic J Environ Agric Food Chem 2011; 10(Pt1): 1858-64.

Shimoyamada M, Ikedo S, Ootsubo R, Watanabe K. Effects of soybean saponins on chymotryptic hydrolyses of soybean proteins. J Agric Food Chem 1998; 46(Pt 12): 4793-7. http://dx.doi.org/10.1021/jf980694j

Potter SM, Jimenez-Flores R, Pollack J, Lone TA, Berber-jimenez MD. Protein saponin interaction and its influence on blood lipids. J Agric Food Chem 1993; 41: 1287-91. http://dx.doi.org/10.1021/jf00032a023




How to Cite

Adegbehingbe Kehinde Tope, & Fakoya Soji. (2013). Effect of Fermentation on Nutrient and Antinutrient Contents of Cocoyam Corm. Journal of Pharmacy and Nutrition Sciences, 3(3), 171–177. https://doi.org/10.6000/1927-5951.2013.03.03.1