Inhibitory Effects of the Edible Mushroom Flammulina velutipes on Lipid Accumulation in 3T3-L1 Cells
DOI:
https://doi.org/10.6000/1927-5951.2013.03.04.1Keywords:
Flammulina velutipes, ergosterol peroxide, 3T3-L1 cells, lipid accumulation.Abstract
We investigated the inhibitory effect of the edible mushroom Flammulina velutipes on intracellular lipid accumulation. Among the five fractions of the F. velutipes extract, the acetone fraction had an inhibitory effect on intracellular lipid accumulation. Seven known compounds were isolated from the acetone fraction: methyl linoleate, methyl γ-linolenate, ergosterol, ergosta-5,7-dien-3β-ol, ergost-7-en-3β-ol, ergosterol peroxide, and 5,6-epoxy-24(R)-methylcholesta-7,22-dien-3β-ol. Ergosterol peroxide exhibited a potent inhibitory effect on the intracellular lipid accumulation. Our results indicate that ergosterol peroxide may be important as an anti-obesity agent since it inhibits the metabolic syndrome.
References
Sanchez C. Modern aspects of mushroom culture technology. Appl Microbiol Biotechnol 2004; 64: 756-62.
Mallavadhani UV, Sudhakar AVS, Satyanarayana KVS, Mahapatra A, Li W, Breemen RB. Chemical and analytical screening of some edible mushrooms. Food Chem 2006; 95: 58-64.
Smiderle FR, Carbonero ER, Mellinger CG, Sassaki GL, Gorin PAJ, Iacomini M. Structural characterization of a polysaccharide and a ?-glucan isolated from the edible mushroom Flammulina velutipes. Phytochemistry 2006; 67: 2189-96.
Cai H, Liu X, Chen Z, Liao S, Zou Y. Isolation, purification and identification of nine chemical compounds from Flammulina velutipes fruiting bodies. Food Chem 2013; 141: 2873-9.
Smiderle FR, Carbonero ER, Sassaki GL, Gorin PAJ, Iacomini M. Characterization of a heterogalactan: some nutritional values of the edible mushroom Flammulina velutipes. Food Chem 2008; 108: 329-33.
Yi C, Sun C, Tong S et al. Cytotoxic effect of novel Flammulina velutipes sterols and its oral bioavailability via mixed micellar nanoformulation. Int J Pharm 2013; 448: 44-50.
Otagiri K, Ohkuma T, Ikekawa T, Tanaka S. Intensification of antitumor-immunity by protein-bound polysaccharide, EA6, derived from Flammulina velutipes (Curt. ex Fr.) Sing. combined with murine leukemia L1210 vaccine in animal experiments. J Pharmacobiodyn 1983; 6: 96-104.
Ko JL, Hsu CI, Lin RH, Kao CL, Lin JY. A new fungal immunomodulatory protein, FIP-five isolated from the edible mushroom, Flammulina velutipes and its complete amino acid sequence. Eur J Biochem 1995; 228: 244-9.
Leung MYK, Fung KP, Choy YM. The isolation and characterization of an immunomodulatory and anti-tumor polysaccharide preparation from Flammulina velutipes. Immunopharmacology 1997; 35: 255-63.
Magae Y, Hayashi N. Double-stranded RNA and virus-like particles in the edible basidiomycete Flammulina velutipes (Enokitake). FEMS Microbiol Lett 1999; 180: 331-5.
Wang HX, Ng TB. Flammulin: a novel ribosome-inactivating protein from fruiting bodies of the winter mushroom Flammulina velutipes. Biochem Cell Biol 2000; 78: 699-702.
Wang H, Ng TB. Isolation and characterization of velutin, a novel low-molecular-weight ribosome-inactivating protein from winter mushroom (Flammulina velutipes) fruiting bodies. Life Sci 2001; 68: 2151-8.
Yamaguchi A, Taira T, Eguchi F. Effect of “Flammulina velutipes extracts” on decrease the lipid accumulation using rat visceral adipocyte culture system. Mushroom Sci Biotechnol 2010; 18: 145-8.
Bao HND, Ochiai Y, Ohshima T. Antioxidative activities of hydrophilic extracts prepared from the fruiting body and spent culture medium of Flammulina velutipes. Bioresour Technol 2010; 101: 6248-55.
Fukushima M, Ohashi T, Fujiwara Y, Sonoyama K, Nakano M. Cholesterol-lowering effects of maitake (Grifola frondosa) fiber, shiitake (Lentinus edodes) fiber, and enokitake (Flammulina velutipes) fiber in rats. Exp Biol Med 2001; 226: 758-65.
Kopelman PG. Obesity as a medical problem. Nature 2000; 404: 635-43.
Mann CC. Public health: provocative study says obesity may reduce U.S. life expectancy. Science 2005; 307: 1716-7.
Ito M, Ito J, Kitazawa H et al. (-)-Ternatin inhibits adipogenesis and lipid metabolism in 3T3-L1 cells. Peptides 2009; 30: 1074-81.
Furuyashiki T, Nagayasu H, Aoki Y et al. Tea catechin suppresses adipocyte differentiation accompanied by down-regulation of PPAR?2 and C/EBP? in 3T3-L1 cells. Biosci Biotechnol Biochem 2004; 68: 2353-9.
Ahn J, Lee H, Kim S, Park J, Ha T. The anti-obesity effect of quercetin is mediated by the AMPK and MAPK signaling pathways. Biochem Biophys Res Commun 2008; 373: 597-608.
Wada T, Ihunnah CA, Gao J et al. Estrogen sulfotransferase inhibits adipocyte differentiation. Mol Endocrinol 2011; 25: 1612-23.
Yahagi T, Daikonya A, Kitanaka S. Flavonol acylglycosides from flower of Albizia julibrissin and their inhibitory effects on lipid accumulation in 3T3-L1 cells. Chem Pharm Bull 2012; 60: 129-36.
Sillerud LO, Han CH, Bitensky MW, Francendese AA. Metabolism and structure of triacylglycerols in rat epididymal fat pad adipocytes determined by 13C nuclear magnetic resonance. J Biol Chem 1986; 261: 4380-8.
Lee S-J, Hong S, Kim Gye-W. ?-Linolenic acid methyl ester from Rhizopus oryzae KSD-815 isolated from Nuruk induces apoptosis in prostate cancer LNCaP cells. J Korean Soc Appl Biol Chem 2010; 53: 752-60.
Shirane N, Takenaka H, Ueda K, Hashimoto Y, Katoh K, Ishii H. Sterol analysis of DMI-resistant and -sensitive strains of Venturia inaequalis. Phytochemistry 1996; 41: 1301-8.
Kobori M, Yoshida M, Ohnishi-Kameyama M, Takei T, Shinmoto H. 5?,8?-Epidioxy-22E-ergosta-6,9(11),22-trien-3?-ol from an edible mushroom suppresses growth of HL60 leukemia and HT29 colon adenocarcinoma cells. Biol Pharm Bull 2006; 29: 755-9.
Bok Jin W, Lermer L, Chilton J, Klingeman Hans G, Towers GHN. Antitumor sterols from the mycelia of Cordyceps sinensis. Phytochemistry 1999; 51: 891-8.
Huang C, Zhang Y, Gong Z et al. Berberine inhibits 3T3-L1 adipocyte differentiation through the PPAR? pathway. Biochem Biophys Res Commun 2006; 348: 571-8.
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Copyright (c) 2013 Yasuhito Nobushi, Yuki Hamada , Ken Yasukawa
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